Genekam Biotechnology AG aus Duisburg auf der MEDICA 2019 in Düsseldorf -- COMPAMED Messe
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Genekam Biotechnology AG

Duissernstr. 65A, 47058 Duisburg
Deutschland
Telefon +49 203 5558580
Fax +49-203 358299
query@genekam.de

Hallenplan

MEDICA 2019 Hallenplan (Halle 3): Stand F05

Geländeplan

MEDICA 2019 Geländeplan: Halle 3

Unsere Produkte

Produktkategorie: Polymerase chain reaction (PCR)

Human T-Cell (CD3 positive cells ) magnetic beads Isolation kit

Human T-Cell (CD3 positive cells ) magnetic beads Isolation kit SB0151

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Produktkategorie: Polymerase chain reaction (PCR)

Human B-Cell (CD19 positive cells ) magnetic beads Isolation kit

Human B-Cell (CD19 positive cells ) magnetic beads Isolation kit SB0152

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Produktkategorie: Polymerase chain reaction (PCR)

Human B-Cell (CD20 positive cells ) magnetic beads Isolation kit

Human B-Cell (CD20 positive cells ) magnetic beads Isolation kit SB0153

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Produktkategorie: Polymerase chain reaction (PCR)

Human B-Cell (CD27 positive cells ) magnetic beads Isolation kit

Human B-Cell (CD27 positive cells ) magnetic beads Isolation kit SB0154

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Produktkategorie: Polymerase chain reaction (PCR)

Erythrocyte removal kit

                           
       +++++  Remove intact erythrocytes from pellet to get pure cell population with magnet: simple, quick, fast and cheap ++++

A. During isolation of cells like mononuclear cells from different sources, many times some of erythrocytes remain in the pellet as unwanted effect. These small number of erythrocytes are problem as there is hardly any method to remove them quickly from bone marrow, tissue as well as pathological lesion. Even to isolate the erythrocytes from the solutions containing circulating tumour cell populations from blood, bone marrow as well as lymphnode biopsies. There are groups working on CAR T-cell therapies, removal of all erythrocytes should be needed to have pure population of mononuclear cells. Similarly during stem cells isolation from umbilical cord blood, erythrocytes may be needed to be removed. There are many more examples e.g. from isolation of particular cell population from umbilical cord wall, the erythrocytes needed to be removed. These are examples, where these erythrocytes are needed to be removed. There are also many applications, where user needs intact erythrocytes to conduct the microscopic analysis as in case of erythrocyte invading pathogens like plasmodium, anaplasma, babesia etc. Genekam has developed the method to isolate these intact erythrocytes with magnetic field, hence one can get erythrocyte free pellet to do further analysis. There will be hardly any lysis of erythrocytes because they will be removed as intact cell population. This kit is very simple to be used (just add Genekam solution, keep for a few minutes and remove the erythrocytes with magnetic field).

This kit is called Genekam erythrocytes removal kit. This kit may be also called erythrocyte separation kit. The user applies magnet to remove the treated erythrocytes. This kit comes as 50X solution, which will be added in PBS to make the needed final concentration. This is a sterile product!

Genekam erythrocyte removal kit (50X) 100 ml SB0147S Euro 100,-
Genekam erythrocyte removal kit (50X) 200 ml SB0147M Euro 180,-

Genekam erythrocyte removal kit (50X) 10 ml SB0147  Euro 30,- as sample kit (shipped only one per client) -- It is a sample kit!

It is 50X solution, hence this kit may be divided into small portions to provide to other users in institute.

The properties and applications of this kit along with the procedure (manual) is as follows:

Storage at 4 °C

Expiry time: 2 years

During isolation of cells e.g. white blood cells populations like mononuclear cells from blood, spleen, umbilical blood cord, bone marrow etc, there are often a problem that erythrocytes are mixing in the population. Genekam has developed a kit to remove the erythrocytes with magnetic rack. This kit has big applications during the development of followings:

1.       immunotherapies like CAR T-cells or dendritic cells

2.       regenerative therapies e.g. isolation of stem cells from umbilical cord blood

3.       regenerative therapies e.g. isolation of CD34 positive cells

4.       nucleic acid isolation e.g. to make pellet erhtrocytes free to isolate the RNA or DNA (where erythrocytes cause inhibition or interference) for PCR, flow cytometry, co focal microscopy as well as next  generation sequencing

5.       to isolate the erythrocytes for detecting the erythrocytes infecting pathogens e.g. Plasmodium, Anaplasma, Babesia etc.

6.  to get the pure cell population from organs like spleen

 

Content:

Genekam Eryhtrocyte removal solution (50X)

Equipment needed:

Magnetic rack

PBS optional

Pipettor

Pipettetips

Centrifuge

Procedure: 1.  Prepare the ready to use solution in washing PBS without any serum or other additive. ( 2ml of Genekam Erythrocyte removal in 98 ml PBS to get ready to use solution because it is 50X): Now make the pellet of isolated cells containing erythrocytes and suspend this pellet in 4-6 ml Genekam erythrocyte removal solution. Keep it at 4 °C for 20 to 30 minutes. Now put the tube in magnetic rack and keep it for 3 minutes. (one will observe that erythrocytes are attractive to the wall through magnetic field). Remove the supernatant from the tube containing the cells free from erythrocytes with pipettor carefully. The pellet or spot containing erythrocytes can be seen as a spot on the wall of the tube because the magnet has attracted the treated erythrocytes. There should be no lysis of erythrocytes!

Hint: If user wants to conduct microscope analysis of erythrocytes containing pathogens. The erythrocytes can be collected as separate population. The erythrocytes can be placed on the microscopic slide for further analysis.

2. Now centrifuge the pellet 2000 rpm for 10 minutes to get the pellet containing cells without erythrocytes.

3. Wash the pellet with PBS and centrifuge it as step 2. This pellet can be used for different applications. 

B. Genekam MNC isolator can be used with above said method to isolate mononuclear cells in large quantity directly from blood. These MNC population can be used for to develop CAR T-cell therapies, other theapies as well as cell culturing.                                                 

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Produktkategorie: Polymerase chain reaction (PCR)

Endotoxin removal kit

++++++++ Endotoxins: Problem during cell cultures, E coli proteins, biopharmaceuticals, vaccines, medical devices and fermentation process+++++
++++++++ Genekam innovative solutions for endotoxin removal+++ Genekam is going to use them in her development of biologicals (human therapeutic antibodies) +++ Try Genekam kit as it is some thing special!!!!

Genekam endotoxin removal Kit (to remove endotoxin): There are many applications, where endotoxins are causing serious problems. Many groups from institutes, universities and biotech industry are working on recombinant proteins expressed in bacteria like E. coli and othe organisms, hence the isolated proteins from much cultures contain a lot of lipopolysaccharides (LPS) so called endotoxins, which can alter the results of isolated proteins during their applications, therefore there is an urgent need to remove them before applying these proteins as therapeutics, diagnostics reagents (medical devices), antibodies (humanised / other origin), vaccines etc. and it is highly recommended to remove them from recombinant proteins in order to start in vitro, preclinical as well as clinical studies. The presence of endotoxins in injectable compounds may lead to sepsis (septic shock!) resulting in the failure of a good compound. Endotoxins may lead to serious problems during the cell cultures. The source of endotoxin may be water, components as well as sera to be used during the culturing or fermentation process. There are different methods available to remove them so e.g. ultracentrifugation, ultrafiltration, chemical treatment etc. None of the methods is going to remove the endotoxin fully, hence a combination of these methods may be alternative. Chemical treatment of isolated protein may leave traces of the chemical leading to side effects during the therapy or development of therapies. Genekam has developed magnetic beads to remove them from isolated proteins. The method is very simple as one can add magnetic beads to the solution, keeps it for 30 minutes and remove the beads (on which the endoxins bind) with a magnet or magnetic field. Genekam beads are designed in such a way that they will remove the maximum endotoxins in the first attempt. However it may be possible that one has to repeat it to remove the endotoxins depending upon the grade of contamination. Genekam beads can be used to remove the LPS from cell cultures, liquids (including water) etc. Even these beads can be used to remove endotoxins from nucleic acid solutions. At present, Genekam offers to remove the endotoxins from small volume samples. If the user has a need to remove endotoxins from large scale samples, Genekam offer here solutions for large scale user.There are many companies around the world, which are producing recombinant proteins or other products for cell therapies, advanced tissue therapies etc. One should test the products for the presence of endotoxins before applying them for different uses. Even the cell culture media should be tested to meet the lowest possible endotoxin concentrations. Technology of Genekam is of great help. Genekam endotoxin removal kit is a sterile product, hence it can be used in sterile applications like cell cultures and sterile proteins (to be used for in vivo research applications). One can regenerate used magnetic beads to be used again. There are many products for which there are legal requirements in EU or USA from FDA, which must be met. During the fermentation process, one uses a number of bioreactors, hence the surface of reactors may be contaminated with biofilms containing endotoxins, we think that one can use Genekam magnetic beads to reduce the load of endotoxins after chemical or mechanical treatment of these reactors. Even Genekam magnetic beads may be the part of the mechanical treatment of bioreactors. These beads can be used to remove with magnetic field or with water from bioreactors. Glasswares may be one of major source of the endotoxins contamination as endotoxins bind on glass surface very strongly. Heating does not remove the endotoxin fully, therefore Genekam magnetic beads should tried to remove them too. Tip for biopharmaceutical industry: Genekam carries huge number of tests for detecting the pathogens during the fermentation process and Genekam has developed a number of products e.g. Mycoplasma removal. Please try to use them too.


Properties:

Fast

Simple

Maximum removal of the endotoxin in first attempt

Removal endotoxin from different sources

Sterile

Multiple use of the magnetic beads possible leading to reduce in cost

To remove the endotoxins from protein as well as nucleic acid solutions

Magnetic beads developed specially to remove the endotoxins with special methods and chemicals

Genekam endotoxin removal kit (Sterile):

1 ml Euro 75.- (SB0146A)

5 ml Euro 300.- (SB0146B)

10 ml Euro 500.-(SB0146C)

Ask for large volume!

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Produktkategorie: Polymerase chain reaction (PCR)

Genekam RNA magnetic isolation kit

Genekam RNA magnetic isolation kit  SB0101

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Produktkategorie: Polymerase chain reaction (PCR)

Genekam DNA magnetic isolation kit

Genekam DNA magnetic isolation kit  SB0100

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Produktkategorie: Polymerase chain reaction (PCR)

Genekam Magnetic beads (Genekam GFP cells catcher)

Genekam Magnetic beads (Genekam GFP cells catcher)  SB0180

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Produktkategorie: Polymerase chain reaction (PCR)

Genekam Magnetic beads (Genekam RFP cells catcher)

Genekam Magnetic beads (Genekam RFP cells catcher)

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Produktkategorie: Polymerase chain reaction (PCR)

Genekam Magnetic separator for 3 x different kinds of tubes e.g. 0.2, 1 and 2ml tubes e.g. DNA/RNA isolation

Genekam Magnetic separator for 3 x different kinds of tubes e.g. 0.2, 1 and 2ml tubes e.g. DNA/RNA isolation

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